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Home > API & Intermediate > Diagnostic Agents > Fluorescein-5-Isothiocyanate Manufacturers > 5(6)-Fluorescein Isothiocyanate (FITC)

Fluorescein-5-Isothiocyanate

CAS NO.： 27072-45-3

FOB Price： NEGOTIABLE Get Latest Price

Specification： ≥97% (HPLC)

Documentation： Get COA after inquiry

Lead Time： In Stock

Max Capacity： 500MT/Year

Packing： 25KG/Carton

Product Detail

Properties

5(6)-Fluorescein Isothiocyanate (FITC)

Details:

CAS Number:27072-45-3

Chemical Formula:C₂₁H₁₁NO₅S

Molecular Weight:389.38

Synonymous:5(6)-Fluorescein isothiocyanate, FITC, Fluorescein-5(6)-isothiocyanate

Specifications:

Purity:≥97% (HPLC)

Appearance (color):yellow to orange

Appearance (form):powder

Solubility:acetone – 1 mg/mL, ethanol – 20 mg/mL

Properties

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. A succinimidyl-ester functional group attached to the fluorescein core, creating "NHS-fluorescein", forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. FITC has excitation and emission spectrum peak wavelengths of approximately 495nm/519nm. Like most fluorochromes, it is prone to photobleaching. Because of the problem with photobleaching, derivatives of fluorescein such as Alexa 488 and DyLight 488 have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.

CAS No.

27072-45-3

Formula

C21H11NO5S

Molecular Mass

389.38100

Exact Mass

389.03600

PSA

120.44000

Logp

4.40010

IUPAC

3',6'-dihydroxy-6-isothiocyanatospiro[2-benzofuran-3,9'-xanthene]-1-one

Matter Classification

organic

Category

Melting Point

>360 °C(lit.)

Density

1.54g/cm3

Boiling Point

708.6ºC at 760mmHg

Flash Point

382.4ºC

Appearance

powder

HS Code

2932190090

UN No.

0.0

DG-Class

0.0

Risk Code

42/43-36/37/38-20/21/22-42

Safety Label

Xn

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